As I pointed out, the text for No. 53 Worthy is the Lamb, Amen is from Revelation 5:12-14. The "amen" is sung as a fugue by the four voices, and I think this is meant to represent part of verse 14: "And the four living creatures said, 'Amen!'" It's as if each voice (soprano, alto, tenor, and bass) is one of the four living creatures.